Service: Aquatic toxicity (Single concentration test, LC 50)

ATRC Laboratory Services for website
Aquatic toxicity (Single concentration test, LC 50)
Daphnia-Acute Lethality Water (48 hr) (EPS 1/RM/11, EPS 1/RM/14)
Trout LC50-Acute Lethality (96hr) (EPS 1/RM/9; EPS 1/RM/13)
You might be interested in the service if you:
  • are overseeing an environmental management unit at an industrial facility (a pulp and paper mill for example),
  • are responsible for ensuring your industrial facility is meeting Effluent Monitoring and Effluent Limits.
Where samples typically come from
  • Effluent generated from industrial facilities, including pulp and paper mills.

How you need to prepare the sample before submission. Instructions on sample submission
The laboratory provides pails and other materials for shipping samples, so clients are encouraged to contact us to coordinate with the lab.
Sample required
Twenty-five litres of sample is required for a single concentration test and eighty litres is required for determination of an LC50. One litre is required for Daphnia.
Each client is provided with pail packages which consist of,
  • a 25L polypropylene plastic pail and lid,
  • a  polyethylene bag,
  • a label,
  • an elastic band,
  • analysis requisition / chain of custody form,
  • 2 CUSTODY SEAL labels
Sampling Instructions
  1. The polyethylene bag lines the polypropylene pail.
  2. The sample is collected by the appropriate sample collection method in the polyethylene lining
  3. Once sampling is complete, the excess air is removed from the bag and the bag is sealed closed with an elastic band.
  4. The pail is covered with the lid. Two custody seals (signed and dated) are placed across the lid to cover the lid and pail. The two seals should be at 180 degrees on each side of the pail.
  5. The label is completed and placed on the exterior of the pail.
  6. The sample is ready for shipment.
For the single concentration test of rainbow trout, or rainbow trout and Daphnia magna, the client is sent of pail package. For LC50 of rainbow trout and Daphnia magna the client is sent one set which consists of three or four pail packages.
It is desirable to keep samples refrigerated during transport. Samples may be transported at ambient temperatures provided ambient temperatures are greater than 1 C and less than 30 C, and that transport time is no longer than 2 days. If these conditions are not met, samples must be maintained in a temperature range of 1 C to 8 C during transport and storage. Do not allow samples to freeze.
What happens to your sample after we receive it. Analytical Methods and Technical Information.
We will first dilute the effluent in a tank with water, then we introduce the test organism to the tank. Trout are introduced  for 96 hours  and daphnia for 48 hours. During the test, temperature, pH, dissolved oxygen, and other water characteristics are monitored and maintained. If more than 50% of the test organisms survive after the allotted time, the effluent would be considered within acceptable effluent limits.
Need more information?
Al Smith, Aquatic Toxicology Research Centre
Have questions about this service?
Not sure if this test will help you?
Ready to submit your sample?

LUCAS Lakehead University