Spectrophotometers Facilities

The Instrumentation Laboratory operates two dual beam, research grade, spectrophotometers.

The instruments are used by researchers and students for a variety of colorimetry based tests including phosphorus, sulphate and chlorophyll and have been used to study colouring in gem stones. Pulp and paper chemists have used the instrument for studies of lignin. Both instruments use the Cary WinUV software.



  • Wavelength Range: 175-3300 nm
  • Wavelength Accuracy: 0.1 nm UV-VIS
  • Photometric Accuracy: .003Abs at 1A
  • Photometric Range:
    • 0-2.0 Abs, 0-3.0 with 1.6 attenuator in ref beam
    • 0-6.0 with 3.0 attenuator in ref beam


  • UV-Vis: R928 PMT
  • NIR: Peltier-cooled lead sulfide photocell


  • UV: D2 (deuterium) lamp
  • Vis-NIR: Tungsten halide lamp


Cary Reflectance Attachment
  • Wavelength Range: 250-2500 nm
  • Sphere Diameter: 110 mm

Cary WINUV 3.0 software, Dell Workstation, Windows 2000

Cary 50 UV-VIS


  • Wavelength range: 190-1100nm
  • Wavelength accuracy: 0.5 nm at 541.9 nm
  • Wavelength reproducibility: 0.1 nm
  • Photometric Range: 3.3 A
  • Photometric accuracy: .005 A at 465 (NIST930)
  • Photometric reproducibility: .004 A at 465 nm


Dual Si diode detectors


Full spectrum Xe pulse lamp

Monochromator: Czerny-Turner 0.25m


  • 10 cm path length cell holder
  • Cary WINUV 3.0 software, Dell Workstation, Windows 2000

Spectrophotometers Theory of Operation

LUCAS Lakehead University