Spectrophotometers Facilities


The Instrumentation Laboratory operates two dual beam, research grade, spectrophotometers.

The instruments are used by researchers and students for a variety of colorimetry based tests including phosphorus, sulphate and chlorophyll and have been used to study colouring in gem stones. Pulp and paper chemists have used the instrument for studies of lignin. Both instruments use the Cary WinUV software.

Cary5e UV-VIS-NIR

SPECIFICATIONS:

  • Wavelength Range: 175-3300 nm
  • Wavelength Accuracy: 0.1 nm UV-VIS
  • Photometric Accuracy: .003Abs at 1A
  • Photometric Range:
    • 0-2.0 Abs, 0-3.0 with 1.6 attenuator in ref beam
    • 0-6.0 with 3.0 attenuator in ref beam

DETECTORS:

  • UV-Vis: R928 PMT
  • NIR: Peltier-cooled lead sulfide photocell

SOURCES:

  • UV: D2 (deuterium) lamp
  • Vis-NIR: Tungsten halide lamp

EQUIPMENT:

Cary Reflectance Attachment
  • Wavelength Range: 250-2500 nm
  • Sphere Diameter: 110 mm

Cary WINUV 3.0 software, Dell Workstation, Windows 2000

Cary 50 UV-VIS

SPECIFICATIONS:

  • Wavelength range: 190-1100nm
  • Wavelength accuracy: 0.5 nm at 541.9 nm
  • Wavelength reproducibility: 0.1 nm
  • Photometric Range: 3.3 A
  • Photometric accuracy: .005 A at 465 (NIST930)
  • Photometric reproducibility: .004 A at 465 nm

DETECTOR:

Dual Si diode detectors

SOURCE:

Full spectrum Xe pulse lamp

Monochromator: Czerny-Turner 0.25m

EQUIPMENT:

  • 10 cm path length cell holder
  • Cary WINUV 3.0 software, Dell Workstation, Windows 2000

Spectrophotometers Theory of Operation


LUCAS Lakehead University