Olympus FV300 Confocal Microscope


FEATURES:

  • Digital Resolution: 256x 256 to 2048x2048. Standard acquisition is 1024 x 1024
  • Grey scale: 0-4095
  • 2 channels
  • Z and time slices

EQUIPMENT:

  • Olympus Fluoview FV300 with Fluoview 4.0 Software
  • BX51 Microscope: Bx51TRF BX51 Stand, Trans/Flourescence, trinoc observ tube
  • FV-LTV-U Laser encl with video port
  • Lasers: Argon (10 mW, force air cooled, blue 488nm), HeNe Green(1mW, 543nm) and HeNe Red (10mW 633 nm) 
  • FV300/500-Laser Combiner
  • FV3-PSU Scan unit (FV-SU302): . with PCI/F Card, 
  • 2 galvanometer scanner mirrors, confocal aperatures 60,100,150,200 and 300 um, PMT detector
  • FV-ZMAD Z-axis stepping motor, .05 um resolution
  •  HBO100W/2 Mercury Burner (8229)
  • PC: P3-1Ghz, 512MB Ram, Viewsonic 22" Monitor
  • FVX-UVT-BX Antivibration Platform

LENS:

  • 100x UPLFL  Plan FL NA 1.3, WD .1 (UB535R)
  • 60x PlanApo NA 1.4 oil
  • 40 x UPlanFl NA 0.75

LASERS:

  • FV-LHARB: Argon laser head (10 mw 488nm)
  • FV-LPAR: Ar Power Supply
  • FV-LHHEG: Green HeNe laser head 05-lgp-193
  • FV-LHHER: Red HeNe laser head 05-lhp-991
  • FV-LPHE: HeNe laser power supply
 

FILTER CUBES:

  • WB:Excitation:  U-M640, wide blue, 450-480, Long barrier pass BA515 
  • NG: Excitation:  U-M663, narrow green 530-550, Long barrier pass BA590
  • NU: Excitation:  360-370, Long barrier pass BA420

FILTERS:

  • FV-605BP: 575-630NM Bandpass Emission
  • FV-660LP: 660nm Long pass Emission 
  • FV-530BP: FVX-BA 510-530 Band pass emission 

Documentation

Documention for COMSTAT analysis.pdf

LUCAS Lakehead University