Ion Chromatograph (IC)


The Dionex ICS-1100 is a dual channel (anions and cations) IC and includes:
  • Dionex ICS-1100 Ion Chromatograph with Anion and Cation Self-Regenerating Suppressors (4mm)
  • AS40 Automated Sampler
  • AS14 Column (4 x 250mm)
  • CS12A Column (4 x 250mm)
  • AS-DV Automated Sampler
  • Chromeleon 7.0 - WorkStation

THEORY of OPERATION

Anions:

A sample is introduced into a stream of carbonate-bicarbonate eluent and passed via high pressure though a highly basic ion exchange column where anions are separated based on their relative affinity to the column.

Cations:

A sample is introduced into a stream of 22mN H2SO4 eluent and passed via high pressure through an ion exchange column where cations are separated based on their relative affinity to the column.

Analysis:

The peaks with eluent are passed through self regenerating micromembrane suppressors (anions or cations) where baseline effects from the eluent are reduced. The conductivity of individual peaks is then measured with a conductivity cell.

For more information....www.scimedia.com/chem-ed/sep/lc/ion-chro.htm

AVAILABLE METHODS:

Current methods are for trace analysis in water.:

WICA (Anions-Trace-Water)

WICA1 (Anions-Higher level)

WICC (Cations-Trace-Water)

WICA- Water-Anions by IC

Anion (Cl)Range (ppm)
Phosphate (o-PO4) 0.050...1.00
Chloride (Cl) 0.050...1.00
Nitrite (NO2-N) 0.025...1.0
Nitrite (NO2-N) 0.025...1.0
Nitrate (NO3-N) 0.025...3.2
Sulphate (SO4) 0.250...25.0

Criteria for acceptance of samples are:
  • Samples do not have visible suspended solids and are not cloudy
  • Maximum analytical range < 100 ppm for any analyte.
  • No organic materials, i.e. humic acid. Colored samples may require cleanup before analysis.
  • Sample pH between 0 and 14
Acceptable sample bottles are glass or polyethylene. Store at 4 degC. Holding times recommended by the EPA for trace anions is immediate to 48 hours (sulphate=3 days). Samples must not be frozen.

WICC : Water-Cations by IC

Anion (Cl)Range (ppm)
Sodium (Na) 0.100 - 10.0
Ammonium (NH4-N) 0.010 - 2.50
Potassium (K) 0.100 - 10.0
Magnesium (Mg) 0.100 - 10.0
Calcium (Ca) 0.100 - 10.0

Criteria for acceptance of samples are:
  • Samples do not have visible suspended solids and are not cloudy
  • Maximum analytical range < 100 ppm for any analyte.
  • No organic materials, i.e. humic acid. Colored samples may require cleanup before analysis.
  • Sample pH between 0 and 14
Acceptable sample bottles are polyethylene. Store at cool to 4 C. Glass can cause Na leaching.Filtration of samples through 0.45 um filters and storage at 4 C will stabilize all analytes for 6 weeks, except NH4+ which is stable for 4 days.

LUCAS Lakehead University